ELISA Kits provide a powerful and simple way to measure protein levels in sample types such as serum, plasma, cell lysates, tissue extracts, or urine. They are used to identify the presence of antigens, antibodies, and other soluble proteins such as hormones, growth factors, and receptors. The ELISA technique measures the binding activity of the analyte to a capture antibody coated on a microplate well. The capture antibody is bound by a conjugated detection antibody, which in turn binds to a chemically reactive substrate to generate a colorimetric, fluorescent, or electrochemical signal that can be measured with an ELISA reader. Go here bosterbio.com
Various ELISA methods can be used to detect and quantify a specific analyte: direct, competitive, indirect, and sandwich ELISAs. Direct ELISAs use a single antibody to directly bind the analyte, which is then detected by a conjugated detection antibody that binds to a secondary antibody-enzyme conjugate. This method offers good assay linearity, but may be less sensitive and specific than a sandwich ELISA.
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Competitive ELISAs employ the principle of competitive enzyme immunoassay to identify target proteins by measuring their interactions with biotin-conjugated capture antibodies in a microplate. They are particularly useful for the detection of phosphorylated proteins such as MAPKs, ERK, STAT, and mTOR. This method is also useful for the rapid and accurate quantitation of peptide hormones when compared to a western blot. All Invitrogen ELISA kits are rigorously tested to ensure high-precision and consistent assay results day after day and lot after lot.